Plasmid

Part:BBa_K3602022:Design

Designed by: Flora Fuglsang   Group: iGEM20_SDU-Denmark   (2020-10-23)


Cas12a w. tags, optimized for human cells


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 430
    Illegal PstI site found at 1492
    Illegal PstI site found at 3901
    Illegal PstI site found at 4099
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 430
    Illegal PstI site found at 1492
    Illegal PstI site found at 3901
    Illegal PstI site found at 4099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
    Illegal BglII site found at 2156
    Illegal BglII site found at 2465
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 430
    Illegal PstI site found at 1492
    Illegal PstI site found at 3901
    Illegal PstI site found at 4099
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 430
    Illegal PstI site found at 1492
    Illegal PstI site found at 3901
    Illegal PstI site found at 4099
    Illegal NgoMIV site found at 3700
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 212


Design Notes

This sequence is designed with the 6x His Tag/Twin Strep/SUMO tags for purification


Source

This sequence is from the PhyLife group of the University of Southern Denmark, where it was originally used for human cells.

References